研究興趣

蛋白質組學方法學及其在生物醫學中的應用研究🦁，重點包括以下研究內容：

• 發展低豐度和翻譯後修飾（如棕櫚酰化、糖化等）蛋白質的選擇性分離富集以及定性定量分析新方法；

• 蛋白質組學技術在重大疾病（如病毒性肝炎🧑🏻‍🎄、心血管疾病、癌症等）發生發展機製與診斷中的應用研究🔥。

學習工作經歷

•   2002-9至2005-7      沐鸣开户   博士

• 1999-9至2002-7      中南大學化學化工學院   碩士

• 1995-9至1999-7      中南工業大學沐鸣开户   學士

• 2005-7~至今   沐鸣开户, 講師，副教授

• 2009~2011   美國加州大學洛杉磯分校🧤，訪問學者

授課情況

• 本科生基礎理論課《分析化學》（必修）;

• 本科生基礎實驗課《無機化學和化學分析實驗I和II》、《儀器分析與物理化學實驗》和《分析化學實驗》（必修）.

代表性論文和專著

1. Weiyu Chen, Yufei He, Caiyun Fang*, Haojie Lu*. Rapid and convenient sample treatment method based on the dissolvable polyacrylamide gel for S-acylation proteomics. Analytical Methods, 2024, DOI: 10.1039/d4ay00937a.

2. Guanghui Ji, Yingying Xiong, Yueyue Li, Guoquan Yan, Jun Yao, Caiyun Fang*, Haojie Lu*. Global analysis of N-myristoylation and its heterogeneity by combining N-terminomics and nanographite fluoride-based solid-phase extraction. Talanta, 2024, 276: 126300.

3. Shen, Yuan#; Zheng, Liangliang#; Fang, Caiyun#; Xu, Yaoyao; Wang, Chao; Li, Jintao; Lei, Mingzhu; Yin, Miao; Lu, Haojie*; Lei, Qunying*; Qu, Jia*; ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation, Cell Reports, 2024, 43: 113720.

4. Wu, Roujun; Ji, Guanghui; Chen, Weiyu; Zhang, Lei; Fang, Caiyun*; Lu, Haojie*; Simultaneous and site-specific profiling of heterogeneity and turnover in protein S-acylation by intact S-acylated peptides analysis with a cleavable bioorthogonal tag, Analyst, 2024, 149(4): 1111-1120.

5. Ji, Guanghui, Wu, Roujun; Zhang, Lei; Yao, Jun; Zhang, Cheng; Zhang, Xiaoqin; Liu, Zhiyong; Liu, Yang; Wang, Ting; Fang, Caiyun*; Lu, Haojie*; Global analysis of endogenously intact Sacylated peptides reveals localization differentiation of heterogeneous lipid chains in mammalian cells, Analytical Chemistry, 2023, 95: 13055-13063.

6. Fang, Caiyun*; Zhang, Xiaoqin; Lu, Haojie*; Quantification of protein palmitoylation by cysteine-SILAC, Chapter 5 in SILAC Methods and Protocols,Methods in Molecular Biology (Springer Protocols), 2023 (ISBN978-1-0716-2862-1), Humana Press.

7. Chen, Weiyu; Ji, Guanghui; Wu, Roujun; Fang, Caiyun*; Lu, Haojie*; Mass spectrometry-based candidate substrate and site identification of PTM enzymes, Trends in Analytical Chemistry, 2023, 160: 116991.

8. Wu, Linlin; Fei, Weiwei; Liu, Zhiyong; Zhang, Lei; Fang, Caiyun*; Lu, Haojie*; Specific and Reversible Enrichment of Early-Stage Glycated Proteome Based on Thiazolidine Chemistry and Palladium-Mediated Cleavage, Analytical Chemistry, 2022, 94(13): 5213-5220.

9. Du, Xiaoxian; Lu, Jingtian; Yang, Lijun; Bao, Huimin; Zhang, Lei; Yan, Guoquan; Fang, Caiyun*; Lu, Haojie*; Rapid and Easy Enrichment Strategy for Naturally Acetylated N Termini Based on LysN Digestion and Amine-Reactive Resin Capture, Analytical Chemistry, 2020, 92(12): 8315-8322.

10. Zhang, Xiaoqin; Zhang, Yuting; Fang, Caiyun*; Zhang, Lei; Yang, Pengyuan; Wang, Changchun*; Lu, Haojie*; Ultra-deep palmitoylomics enabled by a dithiodipyridine functionalized magnetic nanoparticles, Analytical Chemistry, 2018, 90(10): 6161-6168.

11. Zhang, Xiaoqin; Zhang, Lei; Ji, Guanghui; Lei, Qunying; Fang, Caiyun*; Lu, Haojie*; Site-Specific Quantification of Protein Palmitoylation by Cysteine-Stable Isotope Metabolic Labeling, Analytical Chemistry, 2018, 90(17): 10543-10550.